19F NMR detection of a fluorine‐labelled enzyme in vivo

FEBS Letters

Brindle, Kevin¹  Williams, Simon-Peter¹  Boulton, Mark¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England

关键词: Phosphoglycerate kinase;    Yeast;    19F NMR;   

DOI  :  10.1016/0014-5793(89)81073-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphoglycerate kinase was fluorine-labelled in the yeast Saccharomyces cerevisiae by inducing enzyme synthesis in the presence of 5-fluorotryptophan. ¹⁹F NMR measurements on the intact cells showed two resonances from the two tryptophan residues in the protein. The chemical shifts of these resonances were sensitive to the metabolic status of the cells.

【 授权许可】

Unknown