Bradykinin inhibits a potassium M‐like current in rat pheochromocytoma PC12 cells

FEBS Letters

Lopez, Hector¹  Marrion, Neil V.¹  Adams, Paul R.¹  Villarroel, Alvaro¹ 

[1] Howard Hughes Medical Institute, Department of Neurobiology and Behavior, SUNY at Stony Brook, Stony Brook, NY 11794-5230, USA

关键词: M-current;    Ca2+ activated potassium current;    Ca2+ imaging;    Protein kinase C;    Bradykinin;    Muscarine;    (PC 12 cell);   

DOI  :  10.1016/0014-5793(89)81057-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the action of bradykinin (BK) on ionic currents in fused pheochromocytoma PC12 cells under voltage-clamp in whole-cell mode, and on intracellular calcium using fura-2. BK induced the development of an outward current associated with an increase in intracellular calcium, followed by inhibition of an M-like current. The outward current was blocked by (+)-tubocurarine, and prevented when the calcium chelator BAPTA or high concentrations of inositol 1,4,5-triphosphate were introduced into the cell, whereas the M-like current and its inhibition by BK remained unaffected. The protein kinase activator phorbol 12,13 dibutyrate partially reduced the M-current. M-current density did not substantially change after prolonged treatment with nerve growth factor.

【 授权许可】

Unknown