Identification by flow cytometry of two distinct rhodamine‐123‐stained mitochondrial populations in rat liver

FEBS Letters

Lopez-Mediavilla, C.¹  Orfao, A.²  Medina, J.M.¹  Gonzalez, M.¹ 

[1] Departamento de Bioquimica y Biologia Molecular, Universidad de Salamanca, Spain;Servicio de Citometria and Departamento de Hematologia del Hospital Clinico, Universidad de Salamanca, Spain

关键词: Mitochondrion;    Rhodamine-123;    Flow cytometry;    rh-123;    rhodamine-123;    WMF;    whole mitochondrial fraction;    M1;    M2;    and M3;    purified mitochondrial fractions;    LFP;    low fluorescence population;    HFP;    high fluorescence population;    SSC;    side scatter (90° angle light scatter);    FCCP;    carbonyl cyanide-p-trifluoromethoxyphenyl hydrazone;   

DOI  :  10.1016/0014-5793(89)81020-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated rat liver mitochondria were split into three fractions of increasing density when applied to a Percoll gradient. NADH-ubiquinone oxidoreductase, succinate dehydrogenase and cytochrome-c oxidase but not F₁-ATPase activities increased with density as well as respiratory rate in state 3 and the respiratory control index. Flow cytometry of mitochondrial density fractions stained with rhodamine-123 revealed the occurrence in each density fraction of two distinct mitochondrial populations with different fluorescence intensity. The high fluorescence population was minor and its proportion decreased with density. The extent of high fluorescence population staining depended on the deenergized state of the mitochondria suggesting that this population represents an immature form of the mitochondria which may develop into a fully functional organelle by the incorporation of structural and/or functional proteins.

【 授权许可】

Unknown