A model for the molecular organization of cytochrome β‐561 in chromaffin granule membranes

FEBS Letters

Konstantinov, A.A.²  Kamensky, Yu.A.²  Arutjunjan, A.M.²  Esposti, M.Degli¹ 

[1] Section of Biochemistry, Dept of Biology, University of Bologna, Bologna, Italy;A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Cytochrome β-561;    Chromaffin granule;    Heme-heme interaction;    Heme ligand;    CD;    CD;    circular dichroism;    MCD;    magnetic circular dichroism;    CGM;    chromaffin granule membranes;    cyt;    cytochrome;   

DOI  :  10.1016/0014-5793(89)81012-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The CD spectrum of reduced cytochrome (cyt.) β-561 in chromaffin granule membranes resembles that of mitochondrial cyt. β₁ and indicates possible heme-heme interaction in the protein. Based on spectroscopic data and analysis of the amino acid sequence, a model of cyt. β-561 is suggested, in which the protein carries two transmembrane-localized hemes, each coordinated by two histidines. The model accounts for the presence of two different forms of cyt. β-561 in chromaffin granule membranes and provides a mechanism of transmembrane electron transfer by this hemoprotein.

【 授权许可】

Unknown