An increase in intracellular free calcium is an early event during differentiation of cultured human keratinocytes

FEBS Letters

Sharpe, G.R.¹  Gillespie, J.I.²  Greenwell, J.R.² 

[1] University Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, England;Department of Physiological Sciences, The Medical School, Framlington Place, The University, Newcastle upon Tyne NE2 4HH, England

关键词: Keratinocyte;    Differentiation;    Ca2+;    intracellular;    Ca2+;    extracellular;   

DOI  :  10.1016/0014-5793(89)81002-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of [Ca²⁺]_o on [Ca²⁺]_i in cultured human keratinocytes was studied using dual-wavelength microspectrofluorometric techniques. The results show that increasing [Ca²⁺]_o from 70 μM to 1 mM causes an early rise in [Ca²⁺]_i complete by 2 h. Heterogeneity within cultures was demonstrated.The [Ca²⁺]_i in spontaneously differentiated cells of low Ca²⁺ cultures was similar to that of Ca²⁺ induced differentiated cells. The increase in [Ca²⁺]_i preceded the morphological changes and growth inhibition induced by increasing [Ca²⁺]_o. These observations are consistent with an increase in [Ca²⁺]_i mediating differentiation of human keratinocytes.

【 授权许可】

Unknown