Evidence that protein B of the thiosulphate‐oxidizing system of Thiobacillus versutus contains a binuclear manganese cluster

FEBS Letters

Kelly, Don P.¹  Karagouni, Amalia¹  Lu, Wei-Ping¹  Chapman, Alan²  Cammack, Richard² 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England;Department of Biochemistry, King's College London, Campden Hill Road, London W8 7AH, England

关键词: Sulfur-oxidizing bacterium;    ESR;    Electron paramagnetic resonance spectroscopy;    Manganese protein;    (Thiobacillus versutus);   

DOI  :  10.1016/0014-5793(89)80967-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Manganese was shown to be an essential trace metal for growth and thiosulphate oxidation by Thiobacillus versutus in chemostat culture. In the thiosulphate-oxidizing enzyme system of T. versutus, protein B was the only component found to contain manganese in significant amounts. When it was examined by electron spin resonance (ESR) spectroscopy, protein B gave a broad, complex spectrum, indicative of the presence of a dimeric manganese cluster, with manganese in the Mn(II) oxidation state.

【 授权许可】

Unknown