Covalent peptide transfer to cell membrane proteins (peptidyl transferase)

FEBS Letters

Najjar, Victor A.¹  Bump, Nancy J.¹ 

[1] Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA

关键词: Tuftsin;    Oligopeptide;    Membrane protein;    Covalent bond;    ATCC;    American type culture collection;    FCS;    fetal calf serum;    DTT;    dithiothreitol;    HL60;    human leukemia cell line;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phosphate buffered saline;    TCA;    trichloroacetic acid;    Hepes;    4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid;   

DOI  :  10.1016/0014-5793(89)80944-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HL60 cells, rabbit peritoneal granulocytes or membrane preparations of these cells incorporate radioactivity when reacted with the radioactive peptide tuftsin [³H Pro³]-Thr-Lys-Pro-Arg. The radioactivity which is not diminished by repeated treatments with TCA and NaOH, is covalently bound to a membrane acceptor protein of 100 kDa. The peptide is not displaced by large concentrations of its constituent amino acids. The acceptor protein is resolved into one labeled peak by gel filtration on Sephadex G-200, Sephacryl S-300 and by SDS-PAGE. Digestion by trypsin and chymotrypsin results in the production of smaller fragments.

【 授权许可】

Unknown