An infrared spectroscopic study of β‐galactosidase structure in aqueous solutions

FEBS Letters

Muga, A.³  Arrondo, J.L.R.³  Bernabeu, C.¹  Castresana, J.³  Goñi, F.M.² 

[1] Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain;Department of Biochemistry, Faculty of Science, University of the Basque Country, PO Box 644, 48080 Bilbao, Spain

关键词: Fourier-transform infrared spectroscopy;    Galactosidase;    β-;    Protein structure;    Secondary structure;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80901-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fourier-transform infrared spectroscopy has been used to elucidate the secondary structure of E. coli β-galactosidase in aqueous solution. The structure of this enzyme was previously unknown above the level of the amino acid sequence. Spectra have been recorded in both H₂O and D₂O media; mutually complementing data are obtained, that provide unambiguous structural information. The results show that β-galactosidase contains 40% β-sheet and 35% α-helical structure, with smaller proportions of random coil (12%) and β-turns (13%).

【 授权许可】

Unknown