Cloning of the pig aminopeptidase N gene

FEBS Letters

Olsen, Jørgen¹  Norén, Ove¹  Sjöström, Hans¹ 

[1] Department of Biochemistry C, The Panum Institute, Blegdamsvej 3, University of Copenhagen, Copenhagen, Denmark

关键词: Aminopeptidase N;    CD13;    Gene structure;    Regulatory element;    Transcription initiation;    Exon/intron organization;    (Pig);   

DOI  :  10.1016/0014-5793(89)81470-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated four λ-phages covering the complete pig aminopeptidase N/CD 13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5′ upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human α₁-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the Functional peptide.

【 授权许可】

Unknown