Bradykinin blocks the action of EGF, but not PDGF, on fibroblast division

FEBS Letters

Larsson, Olle²  Newman, E.Luke¹  Engström, Wilhelm²  Hyldahl, Louise¹  Rees, Anthony R.¹ 

[1] Laboratory of Molecular Biophysics, University of Oxford, South Parks Road, Oxford OX1 3QU, England;Department of Tumour Pathology, Karolinska Hospital, S-104 01 Stockholm, Sweden

关键词: Protein kinase C;    Epidermal growth factor;    Bradykinin;    DNA synthesis;    DMEM;    Dulbecco's modified Eagle's medium;    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    IGF-1;    insulin-like growth factor 1;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(89)81459-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quiescent fibroblasts derived from human fetal lung can be stimulated to reinitiate DNA synthesis by sequential addition of 3 nM IGF-1 and a low concentration (8 pM) of EGF or by continuous exposure to 10% fetal calf serum or 10 ng/ml PDGF. Bradykinin blocks the IGF-1 and EGF-dependent signals without affecting the response to serum or PDGF. it activates protein kinase C and its anti-mitogenic effect is abolished after this kinase has been down-regulated. Bradykinin has no effect on the binding affinity of the EGF receptor whereas phorbol ester induces its ‘transmodulation’ to low affinity.

【 授权许可】

Unknown