Isolation of a cDNA encoding the human GM2 activator protein

FEBS Letters

Klima, H.¹  Quintern, L.E.¹  Schröder, M.¹  Suzuki, K.²  Nakano, T.²  Sandhoff, K.¹  Gärtner, S.¹  Kwon, H.¹ 

[1] Institut für Organische Chemie und Biochemie, Gerhard-Domagk Str. 1, 53 Bonn 1, FRG;Biological Sciences Research Center, Departments of Neurology and Psychiatry, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA

关键词: GM2 activator protein;    GM2 gangliosidosis;    cDNA sequence;   

DOI  :  10.1016/0014-5793(89)81454-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The G_M2 activator protein is a glycolipid-binding protein required for the lysosomal degradation of ganglioside G_M2. A human fibroblast cDNA library was screened with mixtures of oligonucleotide probes corresponding to four different areas of the amino acid sequence. A putative clone (821 bp) which gave positive signals to all four probe mixtures was purified and sequenced. The sequence was colinear with the sequence of 160 amino acids of the mature G_M2 activator protein. Availability of the cDNA clone should facilitate investigation into function of the G_M2 activator protein and also into genetic abnormalities underlying G_M2 gangliosidosis AB variant.

【 授权许可】

Unknown