The H+ ATPase regulatory subunit of Methanococcus thermolithotrophicus: Amplification of an 800 bp fragment by polymerase chain reaction

FEBS Letters

Bernasconi, P.¹  Taiz, L.¹  Rausch, T.¹  Gogarten, J.P.¹ 

[1] Department of Biology, Thimann Laboratories, University of California Santa Cruz, Santa Cruz, CA 95064, USA

关键词: Archaebacteria;    Methanobacteria;    Molecular evolution;    Proton pump;    Vacuolar type H+ ATPase (V-type);   

DOI  :  10.1016/0014-5793(89)81442-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An 800 bp fragment of Methanococcus thermolithotrophicus genomic DNA was amplified by the polymerase chain reaction method using primers designed from conserved regions of the V-type H⁺ ATPase regulatory subunits from the archaebacterium Sulfolobus, and several eukaryotes. Although more than one product was obtained, only one of them had the expected size and was exclusively amplified in the presence of the left and right primers. The DNA and the deduced protein sequences of the putative Methanococcus H⁺ ATPase subunit revealed homology to the corresponding sequences in Sulfolobus and eukaryotes (about 60% identical residues) and a less evident homology to the eubacterial F₁ -ATPase α-subunit (22% identical residues with E. coli).

【 授权许可】

Unknown