Quantitative immunochemical evidence for identical topography of subunits CF0II and CF0I within the photosynthetic ATP‐synthase of spinach chloroplasts

FEBS Letters

Berzborn, Richard J.¹  Otto, Joachim¹ 

[1] Lehrstuhl für Biochemie der Pflanzen, Fakultät für Biologie der Ruhr-Universität Bochum, Postfach 102148, D-4630 Bochum 1, FRG

关键词: Antibody;    Coupling factor;    Photophosphorylation;    Quaternary structure;    Stalk;    α;    β;    γ;    δ;    ϵ;    subunits of CF1 or F1;    IV;    I;    II;    III;    subunits of CF0;    a;    b;    c;    subunits of F0;    chl;    chlorophyll;    ELISA;    enzyme linked immunosorbent assay;    PMS;    phenazine methosulfate;   

DOI  :  10.1016/0014-5793(89)80810-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monospecific polyclonal antisera against the 16 kDa and the 18 kDa polypeptides II and I from the CF₀ moiety of the photosynthetic ATP-synthase in spinach were produced. Inhibition, agglutination and absorption experiments were combined with quantitative determinations in ELISA of residual antibodies and of amounts of CF₀ and CF₁. It follows that subunits CF₀II and CF₀I are membrane anchored, extend into the matrix space, and are partially shielded underneath or within CF₁. These results on the structure suggest that CF₀II and CF₀I are homologous to each other, and in function both homologous to E. coli F₀b; thus CF₀II together with CF₀I would be part of the ‘stalk’.

【 授权许可】

Unknown