Import of a mutant mitochondrial precursor fails to respond to stimulation by a cytosolic factor

FEBS Letters

Randall, Stephen K.¹  Shore, Gordon C.¹ 

[1]Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada

关键词: Mitochondrial biogenesis;    Cytosolic import factor;    Preornithine carbamyltransferase;    CCCP;    carbonylcyanide-m-chlophenyl hydrazone;    HSF;    heat-stable factor;    NEM;    N-ethylmaleimide;    pOCT;    precursor to ornithine carbamyltransferase;    pO-DHFR;    dihydrofolate reductase fused to the signal sequence of pOCT;   

DOI  :  10.1016/0014-5793(89)80796-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Import of the precursor to ornithine carbamyltransferase is stimulated by a partially-purified, NEM-sensitive soluble factor from rabbit reticulocyte lysate. A mutant in which the carboxy-terminal 73 amino acids were deleted, had a sharply reduced response to this factor. The NEM-sensitive, import-stimulating factor interacts with the surface of mitochondria in the absence of precursor protein. Thus reticulocyte lysate contains an NEM-sensitive, import stimulating factor which interacts both with the surface of mitochondria and whose activity appears to be dependent upon the structure of the mature portion of the precursor.

【 授权许可】

Unknown