Effects of iron‐EDTA on uroporphyrinogen oxidation by liver microsomes

FEBS Letters

Jacobs, Judith M.¹  Lambrecht, Richard W.²  Sinclair, Jacqueline F.²  Sinclair, Peter R.² 

[1]Departments of Pharmacology and Biochemistry, Dartmouth Medical School, Hanover, NH 03756, USA

[2]Veterans Administration, White River Junction, VT 05001, and Departments of Pharmacology and Biochemistry, Dartmouth Medical School, Hanover, NH 03756, USA

关键词: Uroporphyrinogen oxidation;    Cytochrome P-450;    Iron;    (Liver);    DMSO;    dimethyl sulfoxide;    DFX;    desferrioxamine mesylate;    MC;    3-methylcholanthrene;    Mes;    4-morpholineethanesulfonic acid;    PCT;    porphyria cutanea tarda;    PIP;    piperonyl butoxide;    TCB;    3;    4;    3′;    4′-tetrachlorobiphenyl;   

DOI  :  10.1016/0014-5793(89)80753-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uroporphyrinogen oxidation by hepatic microsomes from chick embryos or mice pretreated with methylcholanthrene was increased by addition of iron-EDTA. This increase was partially prevented by catalase, mannitol, ketoconazole and piperonyl butoxide, whereas only ketoconazole and piperonyl butoxide inhibited the oxidation in the presence and absence of iron-EDTA. These data suggest that the oxidations of uroporphyrinogen in the presence and absence of added iron occur by different mechanisms.

【 授权许可】

Unknown