Receptor‐independent metabolism of platelet‐activating factor by myelogenous cells

FEBS Letters

Wykle, Robert L.¹  Jacobson, David²  Redman, Jimmy²  Chabot, Marie C.²  O'Flaherty, Joseph T.² 

[1] Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, NC 27103, USA;Department of Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27103, USA

关键词: Platelet-activating factor;    Receptor;    Phospholipid metabolism;    (Polymorphonuclear leukocyte;    HL-60 promyelocyte);    PMN;    polymorphonuclear neutrophil;    PAF;    platelet-activating factor or 1-O-alkyl-2-acetyl-GPC;    GPC;    sn-glycero-3-phosphocholine;    [3H]PAF;    1-O-[9;    10′-3H2]hexadecyl-2-acetyl-GPC;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(89)80751-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human neutrophils incorporate and metabolize platelet-activating factor (PAF). We dissociated these events from PAF binding to its receptors. Cells were pretreated with either pronase, a PAF antagonist (L652731), or excess PAF. This reduced PAF receptor numbers by 70 to almost 100% but had no comparable effect upon the neutrophil's ability to metabolize PAF. Furthermore, HL-60 cells efficiently metabolized, but did not specifically bind, PAF. Thus, PAF receptor availability did not correlate with PAF metabolic capacity and we conclude that myelogenous tissues can process this bioactive ligand by a receptor-independent pathway.

【 授权许可】

Unknown