期刊论文详细信息
FEBS Letters
Antibodies against an inter‐domain segment of polypeptide chain inhibit active‐site coupling in the pyruvate dehydrogenase multienzyme complex
Appella, Ettore1  Ullrich, Stephen J.1  Perham, Richard N.2  Radford, Sheena E.2 
[1] Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA;Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England
关键词: Pyruvate dehydrogenase;    Multienzyme complex;    Synthetic peptide;    Antibody;    Domain;    Active-site coupling;   
DOI  :  10.1016/0014-5793(89)80750-1
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

A synthetic peptide, AAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKA, proved to be an efficient and specific immunogen in rabbits. The amino acid sequence of the peptide is identical to that of the inter-domain region (PEP3) linking the innermost of the three lipoyl domains to the dihydrolipoamide dehydrogenase-binding domain in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase complex of Escherichia coli. Fab fragments from anti-PEP3 antibodies selectively inhibited active-site coupling in the complex without affecting the individual activities of the three component enzymes, highlighting the role of the inter-domain regions as flexible linkers in catalysis.

【 授权许可】

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