Collagenase gene expression in fibroblasts is regulated by a three‐dimensional contact with collagen

FEBS Letters

Krieg, Thomas²  Adelmann-Grill, Bernhard¹  Hatamochi, Atsushi²  Mauch, Cornelia² 

[1] Max-Planck-Institut für Biochemie, Martinsried, FRG;Dermatologische Klinik der Universität München, Frauenlobstraße 9-11, 8000 München 2 FRG

关键词: Collagenase synthesis;    Collagen gel;    Extracellular matrix;    Fibroblast;    PBS;    phosphate-buffered saline;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;   

DOI  :  10.1016/0014-5793(89)80743-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Collagenase activity in fibroblasts is regulated by cytokines and the interaction with the extracellular matrix. In this study we demonstrate that fibroblasts cultured within a three-dimensional collagen gel show a strong induction of collagenase gene expression. In addition to increased de novo synthesis most of the secreted enzyme was found to be activated leading to a high collagenolytic activity and complete degradation of collagen matrices after removal of fetal calf serum. Collagen I gene expression was found to be reduced under these conditions. These data suggest a specific modulation of cellular metabolism in response to contact with a three-dimensional collagenous matrix resulting in the divergent regulation of collagen and collagenase.

【 授权许可】

Unknown