Dextran protection of erythrocytes from low‐pH‐induced hemolysis

FEBS Letters

Nicolau, Claude¹  Arvinte, Tudor¹  Cudd, Amelia¹  Schulz, Barbara¹ 

[1] Biophor Corporation, Texas A&M University Research Park, 100 Research Parkway, College Station, TX 77840 U.S.A.

关键词: Erythrocyte hemolysis;    Membrane defect;    low-pH-induced;    Dextran protection;    au;    arbitrary units;    FD4;    FD10;    FD20;    FD40;    FD70 and FD150;    fluorescein isothiocyanate labeled dextrans of average molecular masses 3.9;    9;    18.9;    40.5;    71.6 and 148.9 kDa;    respectively;    FITC;    fluorescein isothiocyanate;    PBS7.4;    5 mM sodium-phosphate buffer;    145 mM NaCl;    pH 7.4;    RBC;    red blood cells;   

DOI  :  10.1016/0014-5793(89)80741-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low-pH-induced hemolysis of erythrocytes is inhibited by dextrans. The protective effect was observed with dextrans larger than 40 kDa. Electron microscopy showed dextrans of 150 kDa in a tight association with the erythrocyte membrane. These results indicate that dextrans stop the low-pH-induced hemolysis by interacting with the acid-induced defects in the erythrocyte membrane [(1989) Biochim. Biophys. Acta, in press.

【 授权许可】

Unknown