Potentiation by adrenaline of thrombin‐induced elevation of pHi is not essential for synergistic activation of human platelets

FEBS Letters

Tysnes, Ole-Bjørn¹  Holmsen, Holm¹  Cook, Carol A.¹  Steen, Vidar M.¹ 

[1] Department of Biochemistry, University of Bergen, Norway

关键词: Na+/H+ exchange;    PLC activation;    Aggregation;    Secretion;    BCECF;    2;    7-biscarboxyethyl-5(6)-carboxyfluorescein;    AM;    acetooxymethyl ester;    CP;    creatine phosphate;    CPK;    creatine phosphokinase;    EIPA;    ethylisopropylamiloride;    PA;    phosphatidic acid;    pHi;    cytoplasmic pH;    Pi;    inorganic orthophosphate;    PLA2;    phospholipase A2;    PLC;    phospholipase C;   

DOI  :  10.1016/0014-5793(89)80722-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gel-filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pH_i) in BCECF-loaded platelets. This rise in pH_i was strongly inhibited by the Na⁺/H⁺ exchange blocker EIPA. The potentiation by adrenaline of thrombin-induced PLC activation measured as [³²P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin-induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.

【 授权许可】

Unknown