Cloning and expression of a functional fucose‐specific lectin from an orange peel mushroom, Aleuria aurantia

FEBS Letters

Takeuchi, Naomi¹  Ito, Katumi¹  Nagata, Yoshiho²  Kochibe, Naohisa⁴  Hagiwara, Toshihiko¹  Kobata, Akira³  Fukumori, Fumiyasu¹ 

[1] Research Institute, Nichirei Co., Higashimurayama, Tokyo 189, Japan;Department of Agricultural Chemistry, Chiba University, Matsudo 271, Japan;Department of Biochemistry, The Institute of Medical Science, University of Tokyo, Minato, Tokyo 108 Japan;Department of Biology, Faculty of Education, Gunma University, Maebashi 371, Japan

关键词: Lectin;    Cloning;    (Aleuria aurantia);   

DOI  :  10.1016/0014-5793(89)80709-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aleuria aurantia lectin (AAL) shows sugar-binding specificity for L-fucose. A λgt11 expression library was constructed from A. aurantia poly(A) RNA and screened with a polyclonal antiserum directed against AAL. An immunopositive clone carrying 1.3-kb EcoRI fragment was obtained. The fragment encoded AAL, but lacked a nucleotide sequence corresponding to the two amino-terminal amino acids. The 5′-terminal part of the fragment was replaced with a chemically synthesized DNA fragment and inserted into an expression vector to yield a plasmid pKA-1. Escherichia coli carrying pKA-1 expressed functional AAL and the recombinant AAL showed the same immunological properties as those of natural AAL.

【 授权许可】

Unknown