3′‐Fluoro‐3′‐deoxyribonucleoside 5′‐triphosphates: Synthesis and use as terminators of RNA biosynthesis

FEBS Letters

Sviryaeva, Tatiana V.²  Savochkina, Larissa P.²  Kvasyuk, Eugenii I.¹  Poopeiko, Nicolai E.¹  Pricota, Tamara I.¹  Sivets, Grigorii G.¹  Beabealashvilli, Robert S.²  Mikhailopulo, Igor A.¹ 

[1] Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk 220600 USSR;National Cardiology Research Center, Academy of Medical Sciences, Moscow 121552, USSR

关键词: Fluorodeoxyribonucleoside triphosphate;    RNA synthesis;    terminator;    RNA polymerase;    DNA-dependent;    DNA sequencing;   

DOI  :  10.1016/0014-5793(89)80706-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

3′-Fluoro-3′-deoxy-uridine, -cytidine, -adenosine and -guanosine have been synthesized by glycosylation of the corresponding silylated bases with 1-O-acetyl-2,5-di-O-benzoyl-3-fluoro-3-deoxy-D-ribofuranose in the presence of Friedel-Crafts catalysts and were converted to the 5′- triphosphates, NTP(3′-F). It was shown that NTP(3′-F) are terminators of RNA synthesis catalyzed by DNA-dependent RNA polymerase from E. coli and may thus serve as tools for DNA sequencing.

【 授权许可】

Unknown