Sheep brain glutathione reductase: Purification and general properties

FEBS Letters

Acan, N.L.¹  Tezcan, E.F.¹ 

[1] Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey

关键词: Glutathione reductase;    Enzyme characterization;    (Sheep brain);    GSSGR;    glutathione reductase;    GSSG;    oxidized glutathione;    GSH;    reduced glutathione;   

DOI  :  10.1016/0014-5793(89)80687-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sheep brain glutathione reductase was purified about 11000-fold with an overall yield of 40%. The method included ammonium sulphate fractionation, heat denaturation, 2′,5′-ADP Sepharose 4B and Sephadex G-200 chromatography steps. Specific activity at the final step was 193 IU/mg. The M _r of the enzyme was found to be 116000 by gel filtration chromatography. On SDS-PAGE, two identical subunits of M _r 64000 were obtained. From the spectral data, about 2 mol FAD per mol of enzyme were calculated.

【 授权许可】

Unknown