The gate of mitochondrial porin channel is controlled by a number of negative and positive charges

FEBS Letters

Mirzabekov, Tajib A.¹  Ermishkin, Lev N.¹ 

[1] Institute of Biological Physics, Acad. Sci. USSR, 142292 Pushchino, Moscow Region, USSR

关键词: Porin channel;    Gating charge;    Amidation;    (Mitochondria);   

DOI  :  10.1016/0014-5793(89)80662-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Negatively charged carboxyl groups of mitochondrial porin have been converted into positively charged ones by means of reaction with water-soluble carbodiimide in the presence of ethylenediamine. Properties of channels formed in a planar lipid bilayer by native and modified porins are compared. Amidation has only little influence on the porin channel-forming activity as well as on the open-state conductance of the channel. However, the modification results in a significant enhancement of the voltage dependence of the channel gating and in an increase of the anionic selectivity. It is suggested that the voltage sensor of the porin channel gate is composed of a number of negative (> 14) and positive (> 22) charges.

【 授权许可】

Unknown