Structure of the 5′‐external transcribed spacer of the human ribosomal RNA gene

FEBS Letters

Mazan, Sylvie¹  Bachellerie, Jean-Pierre¹  Renalier, Marie-Hélène¹  Joseph, Nicole¹  Michot, Bernard¹ 

[1] Centre de Recherche de Biochimie et Génétique Cellulaires du CNRS, Université Paul-Sabatier, 118, route de Narbonne, 31062 Toulouse Cédex, France

关键词: rRNA gene;    External transcribed spacer;    Nucleotide sequence;    Secondary structure;    Compositional constraint;    Dinucleotide frequency;   

DOI  :  10.1016/0014-5793(89)80641-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the complete nucleotide sequence of the 3627 bp long 5′-external transcribed spacer (ETS) of a human ribosomal RNA gene. This sequence exhibits only very limited homologies with its mouse counterpart, the only other mammalian specimen analyzed so far. It has very peculiar compositional characteristics, with a highly biased base content (very rich in G+C, very poor in A) and also some very strong dinucleotide preferences. Interestingly, these specific features are shared by the mouse sequence, despite the extensive sequence divergence, and also apply to the other transcribed spacers of mammals indicating that a common and strong structural constraint is exerted on all these regions of the ribosomal gene. An outstanding secondary structure can be formed within the human ETS RNA, which could have a significant role in preribosome assembly.

【 授权许可】

Unknown