αB‐Crystallin is expressed in kidney epithelial cell lines and not in fibroblasts

FEBS Letters

Bhat, Suraj P.¹  Nagineni, Chandrasekharam N.¹ 

[1] Jules Stein Eye Institute, University of California, Los Angeles, School of Medicine, Los Angeles, CA 90024-1771, USA

关键词: Epithelial cell;    Fibroblast;    Crystallin;    αB-;   

DOI  :  10.1016/0014-5793(89)80022-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently shown the presence of αB-crystallin in non-ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an αB-crystallin-specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping with Staphylococcus aureus protease, we demonstrate differential expression of αB-crystallin in epithelial and fibroblast cell lines. αB-Crystallin was detectable only in epithelial cell lines such as MDBK, MDCK, LLCPK₁ and JTC-12, and was not observed in two kidney fibroblast cell lines, one skin fibroblast cell line, and one corneal fibroblast cell line. Differential expression of the αB-crystallin gene was also confirmed by Northern blot analysis of the RNAs isolated from these cell lines. These data suggest a cell-type-specific role for αB.

【 授权许可】

Unknown