Structure of the rat liver ribosome 40 S subunit: Freeze‐drying and high‐resolution shadow casting

FEBS Letters

Vasiliev, V.D.²  Bielka, H.³  Westermann, P.³  Selivanova, O.M.²  Lutsch, G.¹ 

[1] Department of Electron Microscopy, Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch, GDR;Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region, USSR;Department of Cell Physiology, Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch, GDR

关键词: Eukaryotic ribosome;    Subunit structure;    small;    Handedness determination;    Electron microscopy;   

DOI  :  10.1016/0014-5793(89)80438-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Small ribosomal subunits from rat liver have been studied by electron microscopy using freeze-drying and high-resolution shadow casting. The absolute hand of the asymmetric subunit has been determined and its three-dimensional model with a ‘right’ location of the side protuberance has been constructed. The results evidence that pro- and eukaryotic ribosomes have a unique and principally similar structural organization.

【 授权许可】

Unknown