Involvement of tryptase‐related cellular protease(s) in human immunodeficiency virus type 1 infection

FEBS Letters

Koito, Atsushi¹  Katunuma, Nobuhiko²  Takatsuki, Kiyoshi¹  Hattori, Toshio²  Kido, Hiroshi² 

[1] Second Department of Internal Medicine, Kumamoto University Medical School, Kumamoto 860, Japan;Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan

关键词: HIV-1;    Trypstatin;    Neutralization;    Tryptase;   

DOI  :  10.1016/0014-5793(89)80429-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.

【 授权许可】

Unknown