FEBS Letters | |
Involvement of tryptase‐related cellular protease(s) in human immunodeficiency virus type 1 infection | |
Koito, Atsushi1  Katunuma, Nobuhiko2  Takatsuki, Kiyoshi1  Hattori, Toshio2  Kido, Hiroshi2  | |
[1] Second Department of Internal Medicine, Kumamoto University Medical School, Kumamoto 860, Japan;Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan | |
关键词: HIV-1; Trypstatin; Neutralization; Tryptase; | |
DOI : 10.1016/0014-5793(89)80429-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 μM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.
【 授权许可】
Unknown
【 预 览 】
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