Catecholamines increase nerve growth factor mRNA content in both mouse astroglial cells and fibroblast cells

FEBS Letters

Sato, Wakao¹  Satoyoshi, Eijiro³  Furukawa, Yoshiko³  Furukawa, Shoei³  Tomioka, Noboru²  Hayashi, Kyozo⁴ 

[1] Institute of Biological Science, Mitsui Pharmaceuticals Inc., Mobara, Chiba 297 Japan;Life Science Laboratories, Mitsui Toatsu Chemicals Inc., Mobara, Chiba 297 Japan;Division of Neuroimmunology, National Institute of Neuroscience, NCNP, Ogawa-Higashi, Kodaira, Tokyo 187, Japan;Department of Pharmaceutics, Gifu Pharmaceutical University, Mitahora-Higashi, Gifu 502, Japan

关键词: Nerve growth factor;    Catecholamine;    Inducer;    Methylcatechol;    4-;    (Fibroblast;    Astroglial cell);    NGF;    nerve growth factor;    EN;    epinephrine;    NE;    norepinephrine;    DA;    3-hydroxytyramine;    PNS;    peripheral nervous system;    CNS;    central nervous system;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    CM;    conditioned medium;    EIA;    enzyme immunoassay;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(89)81391-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous studies have shown that catecholamines increase the nerve growth factor (NGF) content in medium conditioned by mouse L-M fibroblast cells and mouse astroglial cells. In this study, the NGF mRNA levels in these cells were measured by Northern blot analysis. In astroglial cells treated with epinephrine (EN), the cellular NGF mRNA level increased prior to accumulation of NGF in the culture medium. 3-Hydroxytyramine (DA) and norepinephrine (NE) also increased the cellular NGF mRNA content. An increased level of NGF mRNA elicited by EN was also observed in mouse L-M cells. These results indicate that catecholamines enhance NGF synthesis of L-M fibroblast cells and astroglial cells by increasing the cellular content of NGF mRNA. The present results also indicate that the effects of catecholamines are not mediated by adrenergic receptors.

【 授权许可】

Unknown