Galanin inhibits insulin secretion by direct interference with exocytosis

FEBS Letters

Ullrich, Susanne¹  Wollheim, Claes B.¹ 

[1] Division de Biochimie Clinique, Centre Médical Universitaire, 9, avenue de Champel, CH-1211 Genève 4, Switzerland

关键词: Galanin;    Insulin secretion;    Permeabilization;    Pertussis toxin;    Guanine nucleotide;    (RINm5F cell);   

DOI  :  10.1016/0014-5793(89)81379-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electrically permeabilized RINm5F cells were used to study whether galanin inhibits insulin secretion distally to the generation of soluble second messengers. Ca²⁺-induced insulin secretion was inhibited by the neuropeptide in a dose-dependent manner. Galanin appears to act via a G-protein as persussis toxin treatment abolished the effect. GTP (100 μM), GDP (100 μM) and a low dose of GTPγS (10 μM) did not affect galanin-mediated inhibition of secretion. In contrast, at 100 μM, GTPγS attenuated and GDPβS abolished the effect of the peptide. We conclude that galanin inhibits exocytosis directly by a mechanism involving a G-protein.

【 授权许可】

Unknown