Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells

FEBS Letters

Thim, Lars²  Brattain, Michael G.¹  Hoosein, Naseema M.¹  Jørgensen, Klavs H.² 

[1] Bristol-Baylor Laboratory, Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA;Novo Research Institute, Novo Alle, DK-2880 Bagsvaerd, Denmark

关键词: Protein;    pS2;    cyclic AMP level;    Vasoactive intestinal polypeptide;    PSP;    pancreatic spasmolytic polypeptide;    VIP;    vasoactive intestinal polypeptide;    FBS;    fetal bovine serum;    EGF;    epidermal growth factor;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(89)81357-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [³H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [³H]thymidine incorporation of 50–60% occurred at 3–300, μM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at > 1 μM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [³H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

【 授权许可】

Unknown