Trametes versicolor ligninase: Isozyme sequence homology and substrate specificity

FEBS Letters

Lundquist, Knut¹  Jönsson, Leif²  Nyman, Per Olof²  Karlsson, Olov¹ 

[1] Department of Organic Chemistry, Chalmers University of Technology and University of Göteborg, S-412 96 Göteborg, Sweden;Division of Biochemistry, Chemical Center, University of Lund, PO Box 124, S-221 00 Lund, Sweden

关键词: Ligninase isozyme;    Amino acid sequence homology;    Lignin model compound;    (Trametes versicolor);    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/0014-5793(89)81258-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate specificity of three ligninase isozymes from the white-rot fungus Trametes versicolor has been investigated using stereochemically defined synthetic dimeric models for lignin. The isozymes have been found to attack non-phenolic β-O-4 as well as β-1 lignin model compounds. This finding confirms the classification of the isozymes from T. versicolor as ligninases. The amino-terminal residues of the three isozymes from T. versicolor have been determined using Edman degradation. Minor differences found between the sequences suggest the existence of several structural genes for ligninase in T. versicolor. Comparisons have been made with the sequences of three previously reported ligninases from Phanerochaete chrysosporium, another lignin-degrading fungus. One of the sequences from P. chrysosporium is distinctly more similar to the T. versicolor isozymes than to the other two sequences from P. chrysosporium.

【 授权许可】

Unknown