Endothelin increases single‐channel calcium currents in coronary arterial smooth muscle cells

FEBS Letters

Silberberg, Shai D.¹  Poder, Thomas C.¹  Lacerda, Antonio E.¹ 

[1] Department of Physiology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

关键词: Endothelin;    Ca2+ channel;    (Vascular smooth muscle;    Coronary artery);   

DOI  :  10.1016/0014-5793(89)81242-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelin (ET), a newly identified vasoconstrictor peptide produced by endothelial cells, depends on extracellular calcium for its action [(1988) Nature 332, 411–415]. It is not yet known whether the increase in calcium influx induced by ET results from a direct effect on the Ca²⁺ channels or is secondary to a reduction in membrane potential. To address this question, we studied the effects of ET on single-channel calcium currents of freshly dissociated porcine coronary artery smooth muscle cells using the cell-attached mode of the patch-clamp technique. We show that ET increases Cal⁺-channel activity with no effect on channel open time or conductance. The ability of bath-applied ET to increase single-channel calcium currents in the cell-attached mode is evidence that the peptide acts via a second messenger system.

【 授权许可】

Unknown