The developmental regulation of the L2/HNK‐1 and L3 carbohydrate epitopes in mouse brain Evidence for separate control of lipid‐ and protein‐bound epitopes

FEBS Letters

Breen, Kieran C.¹ 

[1] Dept of Immunology, St. Georges Hospital Medical School, Cranmer Terrace, London SW17 0RE, England

关键词: L2/HNK-1;    L3;    Carbohydrate epitope;    Development;    Cell adhesion;    (Mouse brain);   

DOI  :  10.1016/0014-5793(89)81235-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The carbohydrate epitopes L2/HNK-1 and L3 have previously been identified on various neural cell adhesion molecules and have been suggested to play a role in the mediation of cell-cell adhesion. In this study, the developmental expression of the two epitopes in soluble, membrane-bound and chloroform/methanol-extracted fractions of the constituent mouse brain regions was examined by enzyme-linked immunosorbent assay (ELISA). The protein-bound epitopes were shown to be uniformly developmentally regulated, with levels peaking at postnatal day 20 (P20). The epitopes in a crude chloroform/methanol fraction, however, demonstrated a different pattern, with L2 peaking earlier at postnatal day zero (P0). These results suggest a possible interaction between the control of the two pools of the epitope.

【 授权许可】

Unknown