FEBS Letters | |
The developmental regulation of the L2/HNK‐1 and L3 carbohydrate epitopes in mouse brain Evidence for separate control of lipid‐ and protein‐bound epitopes | |
Breen, Kieran C.1  | |
[1] Dept of Immunology, St. Georges Hospital Medical School, Cranmer Terrace, London SW17 0RE, England | |
关键词: L2/HNK-1; L3; Carbohydrate epitope; Development; Cell adhesion; (Mouse brain); | |
DOI : 10.1016/0014-5793(89)81235-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The carbohydrate epitopes L2/HNK-1 and L3 have previously been identified on various neural cell adhesion molecules and have been suggested to play a role in the mediation of cell-cell adhesion. In this study, the developmental expression of the two epitopes in soluble, membrane-bound and chloroform/methanol-extracted fractions of the constituent mouse brain regions was examined by enzyme-linked immunosorbent assay (ELISA). The protein-bound epitopes were shown to be uniformly developmentally regulated, with levels peaking at postnatal day 20 (P20). The epitopes in a crude chloroform/methanol fraction, however, demonstrated a different pattern, with L2 peaking earlier at postnatal day zero (P0). These results suggest a possible interaction between the control of the two pools of the epitope.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020291848ZK.pdf | 328KB | download |