Pyruvate decarboxylase is like acetolactate synthase (ILV2) and not like the pyruvate dehydrogenase E1 subunit

FEBS Letters

Green, Jeremy B.A.¹ 

[1] European Molecular Biology Laboratory, Meyerhofstrasse 1, D6100 Heidelberg, FRG

关键词: Thiamine pyrophosphate;    Sequence homology;    Protein sequence;    Data base;    FASTP;    Data base;    SWISS-PROT;    TPP;    thiamine pyrophosphate;    PDC;    pyruvate decarboxylase;    PDH;    pyruvate dehydrogenase;    POX;    pyruvate oxidase;    ILV;    acetolactate synthase (isoleucine valine biosynthetic enzyme);   

DOI  :  10.1016/0014-5793(89)80241-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein sequences of pyruvate decarboxylase (PDC) derived from cloned yeast (Saccharomyces cerevisiae) and bacterial (Zymomonas mobilis) genes were compared with each other and with sequence databases. Extensive sequence similarities were found between them and with two others: cytochrome-linked pyruvate oxidase from Escherichia coli and acetolactate synthase (ilvI in E. coli; ILV2 gene in S. cerevisiae). All catalyse decarboxylation of pyruvate using thiamine pyrophosphate (TPP) as cofactor. General overall similarity suggests common ancestry for these enzymes. None of the sequences was similar to the E1 component of pyruvate dehydrogenase from E. coli which also decarboxylates pyruvate with the help of TPP.

【 授权许可】

Unknown