期刊论文详细信息
FEBS Letters | |
Isolation and reconstitution of the high‐affinity choline carrier | |
Breer, H.1  Knipper, M.1  Boekhoff, I.1  | |
[1] University Stuttgart-Hohenheim, Institute for Zoophysiology, Garbenstr. 30, 7000 Stuttgart 70, FRG | |
关键词: Choline transporter; Purification; Reconstitution; Synaptosomal membrane; | |
DOI : 10.1016/0014-5793(89)80228-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Monoclonal antibodies, which block the high-affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium-dependent, hemicholinium-sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high-affinity choline transporter.
【 授权许可】
Unknown
【 预 览 】
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