Isolation and reconstitution of the high‐affinity choline carrier

FEBS Letters

Breer, H.¹  Knipper, M.¹  Boekhoff, I.¹ 

[1] University Stuttgart-Hohenheim, Institute for Zoophysiology, Garbenstr. 30, 7000 Stuttgart 70, FRG

关键词: Choline transporter;    Purification;    Reconstitution;    Synaptosomal membrane;   

DOI  :  10.1016/0014-5793(89)80228-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies, which block the high-affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium-dependent, hemicholinium-sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high-affinity choline transporter.

【 授权许可】

Unknown