Identification of the inhibitor of the plasminogen activator as the major protein secreted by endothelial rat liver cells

FEBS Letters

Kuiper, Johan¹  Van Berkel, Theo J.C.¹  Kamps, Jan A.A.M.¹ 

[1] Division of Biopharmaceutics, Center for Bio-Pharmaceutical Sciences, University of Leiden, Sylvius Laboratories, PO Box 9503, 2300 RA Leiden, The Netherlands

关键词: Plasminogen activator inhibitor;    Liver endothelium;    Liver parenchyma;    Kupffer cell;    (Rat liver);   

DOI  :  10.1016/0014-5793(89)80227-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Freshly isolated Kupffer and endothelial liver cells exhibit a rate of ‘de novo’ protein synthesis which is twice as high per mg cell protein as that of parenchymal liver cells and contribute significantly (7.5% and 5.9%, respectively) to total liver protein secretion. In parenchymal cells the main secretory protein is a 68 kDa protein (containing 19% of the secreted radioactivity, presumably albumin). In Kupffer cells a 49 kDa protein contains 8% of the secreted radioactivity, while in endothelial liver cells a 55 kDa protein is the most prominent secretory protein (containing 11% of the secreted radioactivity). By aid of a specific antibody the 55 kDa protein was identified as the inhibitor of the plasminogen activator and in the liver this protein was only secreted by the endothelial cells.

【 授权许可】

Unknown