The effect of ATP, GTP and cAMP on the cGMP‐dependent conductance of the fragments from frog rod plasma membrane

FEBS Letters

Lyubarsky, A.L.¹  Filatov, G.N.¹  Fesenko, E.E.¹  Kolesnikov, S.S.¹  Jainazarov, A.B.¹ 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: Retinal rod;    cyclic GMP;    Cationic channel;    Conductance regulation;    ADP;    adenosine-5′-diphosphate;    AMP;    adenosine-5′-monophosphate;    AMP-PCP;    adenylyl (β;    γ-methylene)diphosphonate;    AMP-PNP;    adenylyl-imidodiphosphate;    cAMP;    adenosine-3′;    5′-monophosphate;    cyclic;    ATP;    adenosine-5′-triphosphate;    8BrcGMP;    8-bromo-guanosine-3′;    5′-monophosphate;    cyclic;    CTP;    cytidine-5′-triphosphate;    GDP;    guanosine-5′-diphosphate;    GMP;    guanosine-5′-monophosphate;    GMP-PNP;    guanylyl-imidodiphosphate;    cGMP;    guanosine-3′;    5′-monophosphate;    cyclic;    GTP;    guanosine-5′-triphosphate;    GTP-γ-S;    guanosine-5′-O-(3-thiotriphosphate);    EC50;    concentration of an agent at which its effect reaches 500% of maximal value;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    ROS;    rod outer segment;    UTP;    uridine-5′-triphosphate;   

DOI  :  10.1016/0014-5793(89)80218-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a ‘patch-clamp’ method in the ‘inside-out’ configuration, ATP, ADP, AMP-PCP and AMP-PNP have been shown to increase the cGMP-dependent component of the rod plasma membrane conductance 2–4-fold and GTP, GDP but not GMP or nonhydrolyzable GTP analogs GMP-PNP and GTP-γ-S to abolish the ATP action. The ATP and GTP effects were observed at [EDTA] = 1 mM when magnesium and calcium ions were absent. In about half of the experiments the cGMP-dependent conductance was shown to be increased by cAMP in the micromolar concentration range by 10–50%, the cAMP action did not depend on the presence of nucleoside triphosphates. In vivo ATP, GTP and cAMP are assumed to modulate the sensitivity of the photoreceptor plasma membrane to cGMP.

【 授权许可】

Unknown