Carbachol‐induced reverse transformation of Chinese hamster ovary cells transfected with and expressing the m5 muscarinic acetylcholine receptor

FEBS Letters

Conklin, Bruce R.¹  Ma, Alice L.¹  Felder, Christian C.¹ 

[1] National Institute of Mental Health, Section on Pharmacology, Laboratory of Cell Biology, Bethesda, MD 20892, USA

关键词: Muscarinic acetylcholine receptor;    Reverse transformation;    Phorbol ester;    (CHO cell);   

DOI  :  10.1016/0014-5793(89)80195-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reverse transformation was induced in Chinese hamster ovary (CHO) cells transfected with and stably expressing the m5 subtype of the muscarinic acetylcholine receptor when stimulated with the muscarinic agonist, carbachol. Atropine, a muscarinic antagonist, blocked the carbachol-stimulated reverse transformation. CHO cells not transfected with the muscarinic receptor did not change with added carbachol. PMA induced reverse transformation without increasing cAMP accumulation in CHO cells. Carbachol, prostaglandin E₂, and cholecystokinin increased cAMP accumulation but only carbachol caused reverse transformation. Carbachol-stimulated cAMP accumulation occurred at a higher concentration (EC₅₀ 10 μM) than did carbachol-stimulated reverse transformation (EC₅₀ 63 nM). Muscarinic m5 acetylcholine receptor transfected into CHO cells can induce reverse transformation which may be independent of cAMP.

【 授权许可】

Unknown