Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE

FEBS Letters

Appella, Ettore¹  Lerman, Michael I.²  Moore, Stephen K.¹  Yuhki, Naoya³  Yoshimura, Teizo²  Leonard, Edward J.² 

[1] Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892, USA;Laboratory of Immunobiology, National Cancer Institute, Frederick, MD 21701, USA;Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21701, USA

关键词: Inflammation;    Chemotaxis;    Macrophage infiltration;    CSF;    colony-stimulating factor;    CTAP;    connective tissue activating peptide;    IFN;    interferon;    IL-1;    interleukin-1;    LPS;    lipopolysaccharide;    MCP-1;    monocyte chemoattractant protein-1;    PBMNL;    peripheral blood mononuclear leukocyte;    PDGF;    platelet-derived growth factor;    PHA;    phytohemagglutinin;    TNF;    tumor necrosis factor;   

DOI  :  10.1016/0014-5793(89)80590-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purpose of this work was to analyze cDNA encoding human monocyte chemoattractant protein-1 (MCP-1), previously isolated from glioma cell line culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell line U-105MG yielded a clone that coded for the entire MCP-1. Nucleotide sequence analysis and comparison with the amino acid sequence of purified MCP-1 showed that the cDNA clone comprises a 53-nucleotide 5′-non-coding region, an open reading frame coding for a 99-residue protein of which the last 76 residues correspond exactly to pure MCP-1, and a 389-nucleotide 3′-untranslated region. The hydrophobicity of the first 23 residues is typical of a signal peptide. Southern blot analysis of human and animal genomic DNA showed that there is a single MCP-1 gene, which is conserved in several primates. MCP-1 mRNA was induced in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL-1, but not by IL-2, TNF, or IFN-γ. Among proteins with similar sequences, the coding regions of MCP-1 and mouse JE show 68% identity. This suggests that MCP-1 is the human homologue of the mouse competence gene JE.

【 授权许可】

Unknown