Acridine orange, an inhibitor of protein kinase C, abolishes insulin and growth hormone stimulation of lipogenesis in rat adipocytes

FEBS Letters

Smal, Jean¹  Kathuria, Satish¹  De Meyts, Pierre¹ 

[1] Department of Diabetes, Endocrinology and Metabolism, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010, USA

关键词: Acridine orange;    Protein kinase C;    Enzyme inhibitor;    Insulin action;    Growth hormone action;    (Rat adipocyte);   

DOI  :  10.1016/0014-5793(89)80584-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To determine whether protein kinase C plays a role in the actions of insulin and growth hormone in rat adipocytes, we tested the effect of acridine orange, a potent inhibitor of kinase C, on the lipogenic activity of both hormones. This compound completely inhibited the effects of insulin, growth hormone and phorbol ester 12-myristate 13-acetate, whereas 9-acridine carboxylic acid, an analog of acridine orange which does not inhibit kinase C, had no effect. Acridine orange did not act through inhibition of hormone binding. These data are consistent with the involvement of kinase C in the action of insulin and growth hormone on lipogenesis in rat fat cells.

【 授权许可】

Unknown