The crystal structure of the three‐iron ferredoxin II from Desulfovibrio gigas

FEBS Letters

Adman, E.T.¹  LeGall, J.²  Jensen, L.H.¹  Sieker, L.C.¹  Kissinger, C.R.¹ 

[1]Department of Biological Structure, University of Washington, Seattle, WA 98195 USA

[2]Department of Biochemistry, University of Georgia, Athens, GA 30602, USA

关键词: Ferredoxin;    Iron-sulfur cluster;    Crystal structure;    Anomalous scattering;    Disulfide bridge;    (Desulfovibrio gigas);   

DOI  :  10.1016/0014-5793(89)80580-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The crystal structure of oxidized ferredoxin II from the sulfate-reducing bacterium Desulfovibrio gigas has been determined and refined at 1.7 Å resolution. The folding of the polypeptide chain is similar to that of the 2[4Fe-4S] ferredoxin in Peptococcus aerogenes, except for an extended helical segment near the C-terminus. The single [3Fe-4S] cluster in D. gigas is similar to a [4Fe-4S] cluster, but lacks one Fe atom and is coordinated to Cys-8, -14 and -50. The side chain of Cys-11 is not bound to the cluster, but is rotated toward the solvent and modified by some, as yet undetermined, chemical group. Cys-18 and Cys-42 form a disulfide bridge. A previously undetected extra amino acid is found after residue 55.

【 授权许可】

Unknown