Isolation and sequencing of a cDNA clone encoding 107 kDa sialoglycoprotein in rat liver lysosomal membranes

FEBS Letters

Himeno, Masaru¹  Kono, Akira²  Sakaki, Yoshiyuki³  Tanaka, Yoshitaka¹  Sasaki, Hiroyuki³  Kato, Keitaro¹  Noguchi, Youichiro¹  Furuno, Koji¹ 

[1] Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan;National Kyushu Cancer Center, Minami-ku, Fukuoka, Japan;Research Laboratory for Genetic Information, Kyushu University, Higashi-ku, Fukuoka, Japan

关键词: Sialoglycoprotein;    107-kDa;    Lysosomal membrane;    cDNA cloning;    Amino acid sequence;    LGP 107;    107-kDa sialoglycoprotein;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)80561-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA for 107 kDa sialoglycoprotein (LGP 107), the major protein component of rat liver lysosomal membranes, was isolated and sequenced. The 1.8 kbp cDNA contained an open reading frame encoding a polypeptide consisting of 386 amino acid residues (M _r 41914). The deduced NH₂-terminal 10-residue sequence is identical with that determined for purified LGP 107. The primary structure deduced for LGP 107 contains 20 potential N-glycosylation sites and exhibits 82.5, 43 and 60% sequence similarities to mouse LAMP-1, chicken LEP 100, and a 120-kDa human lysosomal glycoprotein, respectively. Among these lysosomal glycoproteins, the amino acid sequence of the putative transmembrane segment is highly conserved. Northern blot hybridization analysis identified a single species of LGP 107 mRNA (2.1 kbp in length) in rat liver, kidney, brain, lung, spleen, heart and pancreas, although its level in pancreas was very low.

【 授权许可】

Unknown