Human recombinant interleukin‐ 1α increases biosynthesis of collagenase and hyaluronic acid in cultured human chorionic cells

FEBS Letters

Mori, Yo¹  Katsura, Morimasa¹  Hirakawa, Shun²  Ito, Akira¹ 

[1] Department of Biochemistry, Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan;Department of Obstetrics and Gynecology, School of Medicine, Toho University, Ohmori-nishi, Ohta, Tokyo 143, Japan

关键词: Interleukin- 1α;    recombinant;    Collagenase;    Collagen;    Hyaluronic acid;    (Human chorionic cell);    IL-1;    interleukin-l;    hrIL-1;    human recombinant interleukin-1α;    MEM;    Eagle's minimum essential medium;    FBS;    fetal bovine serum;    PBS(−);    Ca2+- and Mg2+-free phosphate-buffered saline;    HBSS(−);    Ca2+- and Mg2+-free Hank's balanced salt solution;   

DOI  :  10.1016/0014-5793(89)80553-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of human recombinant interleukin-1α (hrIL-1) on biosynthesis of collagenase and glycosaminoglycans was investigated with fibroblast-like cells of human chorionic membrane. hrIL-1 stimulated cells to produce procollagenase in a dose-dependent manner. Furthermore, it similarly accelerated both biosynthesis and secretion of hyaluronic acid in chorionic cells, but did not modulate the biosynthesis of sulfated glycosaminoglycans. Therefore, the relative concentration of hyaluronic acid vs total glycosaminoglycans increased significantly. These results are connected with the decrease in tensile strength observed in ruptured fetal membranes. Thus, it is proposed that IL-1 from effused leukocytes in fetal membranes plays an important role in connective tissue metabolism, especially in premature rupture of membranes with chorioamnionitis.

【 授权许可】

Unknown