Evidence for the monomeric nature of thymosins

FEBS Letters

Felix, A.M.⁴  Heimer, E.P.⁴  Hannappel, E.³  Yialouris, P.P.²  Haritos, A.A.²  Rosemeyer, M.A.¹ 

[1] Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, England;Zoological Laboratory, Faculty of Sciences, University of Athens, GR 157 84 Athens, Greece;Institut für Biochemie, Medizinische Fakultät der Universität Erlangen, Nürnberg, Fahrstrasse 17, 8520 Erlangen, FRG;Peptide Research Department, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA

关键词: Prothymosin α;    Thymosin β4;    Sedimentation equilibrium;    Gel filtration;   

DOI  :  10.1016/0014-5793(89)80547-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

According to gel-filtration experiments, α- and β-thymosins appear to form oligomers, which are 4-5-fold larger than the corresponding polypeptides. However, on analysis by sedimentation equilibrium ultracentrifugation, prothymosin α and thymosin β₄ showed relative molecular masses of 12 800 and 4600, which are close to the values calculated from their amino acid sequences, confirming their existence in solution as discrete monomeric entities.

【 授权许可】

Unknown