Thermodynamic and kinetic control of ATP synthesis in yeast mitochondria: Role of ΔpH

FEBS Letters

Rigoulet, Michel¹  Guerin, Bernard¹  Beauvoit, Bertrand¹ 

[1] Institut de Biologie Cellulaire et de Neurochimie du CNRS, 1 rue Camille Saint-Saens, 33077 Bordeaux Cedex, France

关键词: ATP synthesis;    Phosphate carrier;    Thermodynamic control;    Kinetic control;    ΔpH;    (Yeast mitochondria);   

DOI  :  10.1016/0014-5793(89)80539-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ATP synthesis rate, measured as the variation in external P_i concentration, varied as a linear function of either Δg̃mH⁺ or ΔGpin, in such a manner that the ΔGpin/Δg̃mH⁺ ratio increased while V _ATP increased. We also observed a linear dependence of the flux control coefficient of the P_i carrier on ΔpH. All the results presented can be explained by a relatively large ΔpH drop when V _ATP increases.

【 授权许可】

Unknown