Crystal structure of thermitase from Thermoactinomyces vulgaris at 2.2 Å resolution

FEBS Letters

Kuranova, Inna P.²  Frömmel, Cornelius¹  Teplyakov, Alexei V.²  Harutyunyan, Emil H.²  Höhne, Wolfgang E.¹ 

[1] Institute of Biochemistry, Humboldt University, Hessische Str. 3-4, Berlin, DDR-1040, GDR;Institute of Crystallography, Acad. Sci. USSR, Leninskii pr. 59, Moscow 117333, USSR

关键词: Thermitase;    Serine proteinase;    X-ray structure;    Subtilisin;    SH group;   

DOI  :  10.1016/0014-5793(89)81194-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The crystal structure of thermitase from Thermoactinomyces vulgaris has been determined by X-ray diffraction at 2.2 Å resolution. The structure was solved by a combination of single isomorphous replacement and molecular replacement methods. The structure was refined to a conventional R factor of 0.24 using restrained least square procedures CORELS and PROLSQ. The tertiary structure of thermitase is similar to that of subtilisin BPN′. The greatest differences between these structures are related to the insertions and deletions in the sequence.

【 授权许可】

Unknown