Extracellular adenosine triphosphate activates phospholipase C and mobilizes intracellular calcium in primary cultures of sheep anterior pituitary cells

FEBS Letters

Fine, Jennifer¹  van der Merwe, P.Anton¹  Wakefield, Ian K.¹  Millar, Robert P.¹  Davidson, James S.¹ 

[1] Department of Chemical Pathology, University of Cape Town Medical School, Observatory 7925, South Africa

关键词: ATP;    Ca2+ efflux;    Purinoreceptor;    Inositol phosphate;    (Anterior pituitary);   

DOI  :  10.1016/0014-5793(89)80156-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In primary cultures of sheep anterior pituitary cells extracellular ATP (ED₅₀ 0.4–0.8 μM) stimulated efflux of ⁴⁵Ca²⁺ from a slow-turnover intracellular pool. ADP was also effective whereas AMP and adenosine were not. The ATP effect was not due to cell permeabilization as 100 μM ATP did not elicit efflux of 2-deoxy[³H]glucose metabolites. This ⁴⁵Ca²⁺ mobilization may be mediated by inositol trisphosphate, since ATP (ED₅₀ 1 μM) stimulated inositol phosphate generation. These results demonstrate P₂-purinoceptors in sheep anterior pituitary cells which are coupled to phospholipase C activation and intracellular Ca₂₊ mobilization, and raise the possibility of a regulatory role for extracellular ATP in the anterior pituitary.

【 授权许可】

Unknown