Carnitine cycle in brown adipose tissue mitochondria as a tool for studying the regulatory role of fatty acids in the uncoupling protein function

FEBS Letters

Krasinskaya, Inna P.²  Drahota, Zdenék¹  Ježek, Petr¹  Smirnova, Ira² 

[1] Department of Bioenergetics, A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR;Institute of Physiology, Czechoslovak Academy of Sciences, Vídeńská 1083, 14220 Prague, Czechoslovakia

关键词: Fatty acid uncoupling;    Uncoupling protein;    Brown adipose tissue mitochondria;    H+ channel;    BAT;    brown adipose tissue;    DASMPI;    2-(p-dimethylaminostyryl)-1-methylpyridinium iodide;    FA;    fatty acid(s);    PN;    purine nucleotide;   

DOI  :  10.1016/0014-5793(89)81213-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A concerted function of purine nucleotide (PN) binding and fatty acid (FA) release from the uncoupling protein (UP) resulting in the maximum coupling (potential) of brown adipose tissue (BAT) mitochondria was demonstrated. The uncoupling effect of FA was studied (at 4 mM MgCl₂): 17 nmol oleate per mg protein caused a slight uncoupling with 8.9 mM ATP but with ATP below 3.6 mM almost total uncoupling was achieved. This shows that the PN-controlled gate can be stabilized in the closed conformation (with 8.9 mM ATP), also when FA is bound to UP. The sensitivity of the FA effect to ATP proves that oleate directly interacts with UP. The closed conformation of the H⁺ channel of UP is then abolished by oleate when a lower free ATP concentration is maintained outside.

【 授权许可】

Unknown