N‐terminal sequencing of photosystem II low‐molecular‐mass proteins 5 and 4.1 kDa components of the O2‐evolving core complex from higher plants

FEBS Letters

Inoue, Yorinao¹  Takio, Koji²  Ikeuchi, Masahiko¹ 

[1] Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan;Laboratory for Aging Process, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan

关键词: Chloroplast gene;    O2 evolution;    Photosystem II core complex;    ORF;    open reading frame;    PS II;    photosystem II;    PVDF;    polyvinylidene difluoride;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)80482-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

High resolution gel electrophoresis in the low-molecular-mass region combined with electroblotting using polyvinylidene difluoride membranes enabled us to sequence the low-molecular-mass proteins of photosystem II membrane fragments from spinach and wheat. The determined N-terminal sequences, all showing considerable homology between the two plants, involved two newly determined sequences for the 4.1 kDa protein and one for the 5 kDa proteins. The sequence of the 4.1 kDa protein did not match any part of the chloroplast DNA sequence from tobacco or liverwort, suggesting that it is encoded by the nuclear genome. In contrast, the sequence of the 5 kDa protein matched ORF38, which is located just downstream of psbE and psbF in the chloroplast DNA and is assumed to be co-transcribed with them. These two components were associated with the O₂-evolving core complex. Sequences of other low-molecular-mass proteins confirmed the previous identification as photosystem II components.

【 授权许可】

Unknown